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Methods in Enzymology

Methods in Enzymology Author John N. Abelson
ISBN-10 0120001004
Release 1995
Pages
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Methods in Enzymology on CD-ROM combines indexing with texts to create an information resource that brings single-search access to two database collections.



Cumulative Subject Index to Methods in Enzymology

Cumulative Subject Index  to  Methods in Enzymology Author
ISBN-10 0121820408
Release 1975
Pages
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Cumulative Subject Index to Methods in Enzymology has been writing in one form or another for most of life. You can find so many inspiration from Cumulative Subject Index to Methods in Enzymology also informative, and entertaining. Click DOWNLOAD or Read Online button to get full Cumulative Subject Index to Methods in Enzymology book for free.



Energetics of Biological Macromolecules

Energetics of Biological Macromolecules Author Gary K. Ackers
ISBN-10 012182196X
Release 1998
Pages 548
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General Description of the Volume: The very existence of biological structures and their functional interactions are dictated by energetic relationships. Thus the central theme of this volume is that thermodynamic methods, i.e. techniques that probe the energetics of biological macromolecules, now comprise a powerful and practical family of tools for research in modern biology. The application of thermodynamics and statistical thermodynamics to biochemical and biophysical systems is presented. This volume supplements Methods in Enzymology, Volume 259. General Description of the Series: The critically acclaimed laboratory standard for more than forty years, Methods in Enzymology is one of the most highly respected publications in the field of biochemistry. Since 1955, each volume has been eagerly awaited, frequently consulted, and praised by researchers and reviewers alike. Now with more than 300 volumes (all of them still in print), the series contains much material still relevant today--truly an essential publication for researchers in all fields of life sciences. Key Features * Major topics covered include: * Deciphering rules of helix stability in peptides * Protein Folding in Membranes * Molecular Crowding * Study of the Bohr Effect in Hemoglobin Intermediates * Photoacoustic Calorimetry of Proteins * Theoretical Aspects of Isothermal Titration Calorimetry * Energetic Methods to Study Bifunctional Biotin Repressor



Nucleosomes Histones Chromatin

Nucleosomes  Histones   Chromatin Author Carl Wu
ISBN-10 9780123919403
Release 2012
Pages 337
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This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering nucleosomes, histones and chromatin, it has chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins. Continues the legacy of this premier serial by containing quality chapters authored by leaders in the field The first of 2 volumes covering nucleosomes, histones and chromatin Chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins



Guide to Protein Purification

Guide to Protein Purification Author Richard R. Burgess
ISBN-10 0123745365
Release 2009
Pages 851
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The second edition of this classic Guide to Protein Purification provides a complete update to existing methods in the field, reflecting the enormous advances made in the last two decades. In particular, proteomics, mass spectrometry, and DNA technology have revolutionized the field since the first edition's publication but through all of the advancements, the purification of proteins is still an indispensable first step in understanding their function. This volume examines the most reliable, robust methods for researchers in biochemistry, molecular and cell biology, genetics, pharmacology, biotechnology and sets a standard for best practices in the field. It relates how these traditional and new cutting-edge methods connect to the explosive advancements in the field. This "Guide to" gives imminently practical advice in order to avoid costly mistakes in choosing a method and brings in perspective from the premier researchers while presents a comprehensive overview of the field today. Gathers top global authors from industry, medicine, and research fields across a wide variety of disciplines including biochemistry, genetics, oncology, pharmacology, dermatology and immunology. Assembles chapters on both common and less-common relevant techniques. Provides robust methods as well as an analysis of the advancements in the field that, for an individual investigator, can be a demanding and time-consuming process.



Numerical Computer Methods

Numerical Computer Methods Author
ISBN-10 9780080497211
Release 2004-05-25
Pages 489
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The aim of Numerical Computer Methods, Part D is to brief researchers of the importance of data analysis in enzymology, and of the modern methods that have developed concomitantly with computer hardware. It is also to validate researchers' computer programs with real and synthetic data to ascertain that the results produced are what they expected. Selected Contents: Prediction of protein structure Modeling and studying proteins with molecular dynamics Statistical error in isothermal titration calorimetry Analysis of circular dichroism data Model comparison methods



Thermodynamics and Kinetics of Drug Binding

Thermodynamics and Kinetics of Drug Binding Author György Keserü
ISBN-10 9783527673056
Release 2015-03-09
Pages 360
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This practical reference for medicinal and pharmaceutical chemists combines the theoretical background with modern methods as well as applications from recent lead finding and optimization projects. Divided into two parts on the thermodynamics and kinetics of drug-receptor interaction, the text provides the conceptual and methodological basis for characterizing binding mechanisms for drugs and other bioactive molecules. It covers all currently used methods, from experimental approaches, such as ITC or SPR, right up to the latest computational methods. Case studies of real-life lead or drug development projects are also included so readers can apply the methods learned to their own projects. Finally, the benefits of a thorough binding mode analysis for any drug development project are summarized in an outlook chapter written by the editors.



Enzyme Kinetics

Enzyme Kinetics Author Hans Bisswanger
ISBN-10 9783527806492
Release 2017-06-23
Pages 320
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Now in full color for a more intuitive learning experience, this new edition of the long-selling reference also features a number of new developments in methodology and the application of enzyme kinetics. Starting with a description of ligand binding equilibria, the experienced author goes on to discuss simple and complex enzyme reactions in kinetic terms. Special cases such as membrane-bound and immobilized enzymes are considered, as is the influence of external conditions, such as temperature and pH value. The final part of the book then covers a range of widely used measurement methods and compares their performance and scope of application. With its unique mix of theory and practical advice, this is an invaluable aid for teaching as well as for experimental work.



Macromolecular Crystallography

Macromolecular Crystallography Author Charles W. Carter
ISBN-10 0121827771
Release 1997
Pages 368
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Macromolecular Crystallography has been writing in one form or another for most of life. You can find so many inspiration from Macromolecular Crystallography also informative, and entertaining. Click DOWNLOAD or Read Online button to get full Macromolecular Crystallography book for free.



Osmosensing and Osmosignaling

Osmosensing and Osmosignaling Author
ISBN-10 0080552110
Release 2007-10-01
Pages 632
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For over fifty years the Methods in Enzymology series has been the critically acclaimed laboratory standard and one of the most respected publications in the field of biochemistry. The highly relevant material makes it an essential publication for researchers in all fields of life and related sciences. This volume features articles on the topic of osmosensing and osmosignaling written by experts in the field.



Calorimetry

Calorimetry Author Stefan Mathias Sarge
ISBN-10 9783527649389
Release 2014-02-25
Pages 304
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Clearly divided into three parts, this practical book begins by dealing with all fundamental aspects of calorimetry. The second part looks at the equipment used and new developments. The third and final section provides measurement guidelines in order to obtain the best results. The result is optimized knowledge for users of this technique, supplemented with practical tips and tricks.



The Resolution Revolution Recent Advances In cryoEM

The Resolution Revolution  Recent Advances In cryoEM Author
ISBN-10 9780128054352
Release 2016-08-26
Pages 486
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cryoEM, a new volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods and new developments in recording images, the creation, evaluation and validation of 3D maps from the images, model building into maps and refinement of the resulting atomic structures, and applications of essentially single particle methods to helical structures and to sub-tomogram averaging. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods that determine the structures of biological molecules, a vital step for understanding their function Contains the technical developments underpinning the advances of cryoEM and captures the exciting insights that have resulted



Riboswitch Discovery Structure and Function

Riboswitch Discovery  Structure and Function Author
ISBN-10 9780128013359
Release 2015-12-17
Pages 570
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This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in riboswitch discovery and validation, synthesis and sample prep methods for large RNAs, riboswitch structure and function methods, folding pathways and dynamics, and ligand interactions and thermodynamics. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in riboswitch discovery, structure and function Contains sections on such topics as riboswitch discovery and validation, synthesis and sample prep methods for large RNAs, riboswitch structure and function methods, folding pathways and dynamics, ligand interactions and thermodynamics



Protein Ligand Interactions

Protein Ligand Interactions Author G. Ulrich Nienhaus
ISBN-10 161737525X
Release 2010-10-28
Pages 568
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A readily reproducible collection of established and emerging techniques for studying the interaction between proteins and ligands, including biochemical/bulk techniques, structure analysis, spectroscopy, single-molecule studies, and theoretical/computational tools. Among the highlights are surface plasmon resonance (SPR) and reflectometric biosensor approaches, high-throughput screening with confocal optics microscopy, single molecule fluorescence and fluorescence correlation spectroscopy (FCS), atomic force microscopy (AFM), crystallography of reaction intermediates, and time-resolved x-ray crystallography. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.



Titration Calorimetry

Titration Calorimetry Author Lee D. Hansen
ISBN-10 9783319782508
Release 2018-04-06
Pages 57
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This Brief describes the calibration of titration calorimeters (ITCs) and calculation of stoichiometry, equilibrium constants, enthalpy changes, and rate constants for reactions in solution. A framework/methodology for model development for analysis of ITC data is presented together with methods for assessing the uncertainties in determined parameters and test data sets. This book appeals to beginners, as well as to researchers and professionals in the field.



Isotope Labeling of Biomolecules Applications

Isotope Labeling of Biomolecules     Applications Author
ISBN-10 9780128030745
Release 2016-01-11
Pages 482
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Isotope Labeling of Biomolecules: Applications, the latest in the Methods in Enzymology series, focuses on stable isotope labeling methods and applications for biomolecules. This practical guide to biomolecular labeling looks at new techniques that are becoming widely used. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Focuses on stable isotope labeling of biomolecules, which is important for structural studies of proteins and nucleic acids



Calorimetry in Food Processing

Calorimetry in Food Processing Author Gönül Kaletunç
ISBN-10 9780813805948
Release 2009-08-06
Pages 384
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Calorimetry in Food Processing: Analysis and Design of Food Systems introduces the basic principles of calorimetry and highlights various applications of calorimetry to characterize temperature-induced changes including starch gelatinization and crystallization, lipid transitions, protein denaturation, and inactivation of microorganisms in a variety of food and biological materials. Emphasis is given to the use of calorimetry as a tool for evaluation of processing requirements in order to assess the efficacy of food processing and for characterization of the effects of changes in formulation and processing conditions.