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Colloidal Gold

Colloidal Gold Author
ISBN-10 9780323138819
Release 2012-12-02
Pages 536
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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. Principles, methods, and applications of colloidal gold methodology in cytochemistry and immunochemistry Methods for preparing colloidal gold particles of different sizes Protein A-gold, protein G-gold, and lectin-gold techniques The use of resins and thin cryosections Multiple labeling



Colloidal Gold

Colloidal Gold Author
ISBN-10 9780323139021
Release 2012-12-02
Pages 484
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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. Colloidal gold has become the method of choice in immunocytochemistry and many areas of cell biology. This universal method is applicable to most microscopical systems including optical microscopy; scanning, transmission, and high voltage electron microscopy; photoelectron, photon, fluorescent darkfield, and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This book is among the first available to cover the principles and methodology of colloidal gold in microscopy. Methods are described step by step, to enable researchers to learn these complex procedures solely by reference to these books Problems and limitations of techniques are discussed Guides users to avoid problems and choose the correct procedures for specific applications Contributors are eminent authorities in their fields



Colloidal Gold

Colloidal Gold Author M. A. Hayat
ISBN-10 UOM:39015018313489
Release 1989
Pages 536
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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. This universal method is applicable to most microscopical systems including optical microscopy, scanning, transmission and high voltage electron microscopy, and photoelectron, photon, fluorescent darkfield and epipolarization microscopy. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection.



Colloidal Gold

Colloidal Gold Author M. A. Hayat
ISBN-10 UVA:X002753313
Release 1991
Pages 421
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Since its introduction in 1971, the development and application of colloidal gold as a marker in electron microscopy has been phenomenal. This state-of-the art, multi-volume treatise provides researchers, technicians, teachers, and students with the most comprehensive coverage of the principles and methodology of colloidal gold microscopy available today. Colloidal gold allows high and low resolution studies, enzyme and nucleic acid labeling, study of dynamic cellular processes, and virus detection. This third volume completes Hayat's coverage of the principles and methodology of colloidal gold in microscopy. The three-volume set should become the standard reference in the field. Key Features * Among the first books to cover principles and methodology of colloidal gold in microscopy * Describes methods step by step to enable researchers to learn these complex procedures solely by reference to the book * Discusses problems and limitations of techniques * Guides users to avoid problems and choose the correct procedures for specific applications * Contributors are eminent authorities in their fields Topics covered in Volume 3 include: * Production of monoclonal and polyclonal antibodies * Lowicryl, polar, and apolar resins * Preserving and localizing antigenicity; sectioning; high-resolution morphological localization * Diaminobenzidine-colloidal gold; microinjection of colloidal gold; double labeling with backscattered electrons; microwave irradiation * Applications to biomedical studies * Colloidal gold in conjunction with video-enhanced microscopy



Immunogold Silver Staining

Immunogold Silver Staining Author M. A. Hayat
ISBN-10 0849324491
Release 1995-08-03
Pages 336
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This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.



Colloid Science

Colloid Science Author Terence Cosgrove
ISBN-10 9781444320183
Release 2010-02-16
Pages 394
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Colloidal systems are important across a range of industries, such as the food, pharmaceutical, agrochemical, cosmetics, polymer, paint and oil industries, and form the basis of a wide range of products (eg cosmetics & toiletries, processed foodstuffs and photographic film). A detailed understanding of their formation, control and application is required in those industries, yet many new graduate or postgraduate chemists or chemical engineers have little or no direct experience of colloids. Based on lectures given at the highly successful Bristol Colloid Centre Spring School, Colloid Science: Principles, Methods and Applications provides a thorough introduction to colloid science for industrial chemists, technologists and engineers. Lectures are collated and presented in a coherent and logical text on practical colloid science.



Immunogold Silver Staining

Immunogold Silver Staining Author M. A. Hayat
ISBN-10 0849324491
Release 1995-08-03
Pages 336
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This book discusses the principles, methods, and applications of immunogold-silver staining (IGSS) to biomedical areas. It focuses on the latest advances in the dynamic and progressive field of IGSS.



Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy Author James B. Pawley
ISBN-10 0306448262
Release 1995-01-01
Pages 632
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This newly updated second edition details the latest instrumentation and applications of the confocal microscope. This edition features 21 new chapters and includes information on preparing living specimens for the confocal microscope.



Biological Electron Microscopy

Biological Electron Microscopy Author Michael J. Dykstra
ISBN-10 0306477491
Release 2003-12-31
Pages 534
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Electron microscopy is frequently portrayed as a discipline that stands alone, separated from molecular biology, light microscopy, physiology, and biochemistry, among other disciplines. It is also presented as a technically demanding discipline operating largely in the sphere of "black boxes" and governed by many absolute laws of procedure. At the introductory level, this portrayal does the discipline and the student a disservice. The instrumentation we use is complex, but ultimately understandable and, more importantly, repairable. The procedures we employ for preparing tissues and cells are not totally understood, but enough information is available to allow investigators to make reasonable choices concerning the best techniques to apply to their parti cular problems. There are countless specialized techniques in the field of electron and light microscopy that require the acquisition of specialized knowledge, particularly for interpretation of results (electron tomography and energy dispersive spectroscopy immediately come to mind), but most laboratories possessing the equipment to effect these approaches have specialists to help the casual user. The advent of computer operated electron microscopes has also broadened access to these instruments, allowing users with little technical knowledge about electron microscope design to quickly become operators. This has been a welcome advance, because earlier instru ments required a level of knowledge about electron optics and vacuum systems to produce optimal photographs and to avoid "crashing" the instruments that typically made it difficult for beginners.



Practical Electron Microscopy

Practical Electron Microscopy Author Elaine Evelyn Hunter
ISBN-10 0521385393
Release 1993-09-24
Pages 173
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This is an extensively illustrated laboratory manual of transmission electron microscopy techniques for the laboratory technician, graduate student, or researcher. Chapters begin with a general discussion, move on to the chemicals and equipment required for the method being described and conclude with a step-by-step presentation of the method and instructions for the preparation of solutions. Notes at the end of each chapter warn of possible pitfalls and outline "tricks of the trade". The methods and techniques outlined have been tested for over ten years in clinical and research laboratory situations, and are entirely reliable. Practical Electron Microscopy covers fixation, dehydration and embedding, semithin and thin sectioning, the electron microscope, and photography. For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added, one on immunoelectron microscopy using colloidal gold, and one dealing with such special techniques as retrieving specimens from paraffin and handling nasal brushings and blood samples.



Lateral Flow Immunoassay

Lateral Flow Immunoassay Author Raphael Wong
ISBN-10 1597452408
Release 2008-12-16
Pages 224
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Due to the simplicity, relative accuracy, fast result reporting, and user-friendliness of lateral flow immunoassay, its use has undergone tremendous growth in the diagnostic industry in the last few years. Such technology has been utilized widely and includes pregnancy and woman's health determination, cardiac and emergency conditions monitoring and testing, infectious disease including Flu screening, cancer marker screening, and drugs abuse testing. This book covers the scope of utilization, the principle of the technology, the patent concerns, information on the development and production of the test device and specific applications will be of interest to the diagnostic industry and the general scientific community.



Stains and Cytochemical Methods

Stains and Cytochemical Methods Author M.A. Hayat
ISBN-10 0306442949
Release 1993-03-31
Pages 455
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The author's primary aim is to thoroughly explain the biochemical concepts governing cytochemical procedures for transmission electron microscopy. Such information provides undergraduate and graduate students, technicians, and researchers with a more profound understanding of electron micrographs, as well as the knowledge to refine existing techniques and develop new methodologies.



Analytical Morphology

Analytical Morphology Author Jiang Gu
ISBN-10 0817639578
Release 1997-03-20
Pages 264
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Analytical Morphology is a new discipline dealing with the analysis of cell and tissue components using analytical maneuvers that go beyond those of conventional morphology and histopathology. It utilizes new advances in other disciplines, such as immunology, molecular biology, laser technology, microwave technology and computer science, to highlight cellular components such as peptides, proteins, or nucleotide sequences through chemical, physical, immunological, or molecular biological reactions. These methods include immunocytochemistry, in situ hybridization, in situ PCR, antigen retrieval, image analysis, and the like. The book is intended for morphological scientists, pathologists, advanced students, and technicians who are interested in keeping abreast of this rapidly emerging discipline.



Immuno Gold Electron Microscopy in Virus Diagnosis and Research

Immuno Gold Electron Microscopy in Virus Diagnosis and Research Author Alexander D. Hyatt
ISBN-10 084936759X
Release 1992-11-10
Pages 464
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This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.



Electron Microscopy of Plant Cells

Electron Microscopy of Plant Cells Author C Hawes
ISBN-10 9780323148986
Release 2012-12-02
Pages 476
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Electron Microscopy of Plant Cells serves as manual or reference of major modern techniques used to prepare plant material for transmission and scanning electron microscopy. There have been other books that generally discuss electron microscope methodology. This book focuses on problem areas encountered through the presence of tough cell walls and large central vacuole. It details preparative techniques for botanical specimens. Each of the nine chapters of this book covers the basic principles, useful applications, and reliable procedures used on the method of electron microscopy. Other topics discussed in each chapter include the general preparation and straining of thin sections, quantitative morphological analysis, and enzyme cytochemistry. This book also explains the immunogold labelling, rapid-freezing methods, and ambient- and low-temperature scanning electron microscopy among others. This book will be invaluable to general scientists, biologists, botanists, and students specializing in plant anatomy.



Antibodies in Cell Biology

Antibodies in Cell Biology Author
ISBN-10 0080859356
Release 1993-11-17
Pages 452
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Antibodies in Cell Biology focuses on a new generation of protocols aimed at the cell biologist. This laboratory manual features systems and techniques that are especially relevant for modern problems. The contributing authors have been carefully chosen for their specific expertise, and have provided detailed protocols, recipes, and troubleshooting guides in each chapter. The book is designed for any researcher or student who needs to use antibodies in cell biology and related research areas. Practical applications and future emphases of antibodies, including: Light microscopic immunolocalization of antigens Gold particles in immunoelectron microscopy Special methods of fixation and permeabilization Microinjection of antibodies into living cells Antibodies to identify cDNA clones Antisense antibody strategies



Molecular Methods in Plant Pathology

Molecular Methods in Plant Pathology Author U. S. Singh
ISBN-10 9781351430210
Release 2017-12-14
Pages 544
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Molecular Methods in Plant Pathology covers methods in phytopathology at the molecular level, including PCR techniques, electron microscopy, tissue culturing, and the cloning of disease-resistant genes. Phytopathologists, botanists, horticulturists, and anyone working in agriculture will find this a useful reference on biophysical, biochemical, biomolecular, and biotechnological methods.