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Fundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging Author Douglas B. Murphy
ISBN-10 9781118382936
Release 2012-08-22
Pages 552
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Fundamentals of Light Microscopy and Electronic Imaging, Second Edition provides a coherent introduction to the principles and applications of the integrated optical microscope system, covering both theoretical and practical considerations. It expands and updates discussions of multi-spectral imaging, intensified digital cameras, signal colocalization, and uses of objectives, and offers guidance in the selection of microscopes and electronic cameras, as well as appropriate auxiliary optical systems and fluorescent tags. The book is divided into three sections covering optical principles in diffraction and image formation, basic modes of light microscopy, and components of modern electronic imaging systems and image processing operations. Each chapter introduces relevant theory, followed by descriptions of instrument alignment and image interpretation. This revision includes new chapters on live cell imaging, measurement of protein dynamics, deconvolution microscopy, and interference microscopy. PowerPoint slides of the figures as well as other supplementary materials for instructors are available at a companion website: www.wiley.com/go/murphy/lightmicroscopy



Fundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging Author Douglas B. Murphy
ISBN-10 9780471234296
Release 2002-06-18
Pages 384
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Over the last decade, advances in science and technology have profoundly changed the face of light microscopy. Research scientists need to learn new skills in order to use a modern research microscope–skills such as how to align microscope optics and perform image processing. Fundamentals of Light Microscopy and Electronic Imaging explores the basics of microscope design and use. The comprehensive material discusses the optical principles involved in diffraction and image formation in the light microscope, the basic modes of light microscopy, the components of modern electronic imaging systems, and the image processing operations necessary to acquire and prepare an image. Written in a practical, accessible style, Fundamentals of Light Microscopy and Electronic Imaging reviews such topics as: Illuminators, filters, and isolation of specific wavelengths Phase contrast and differential interference contrast Properties of polarized light and polarization microscopy Fluorescence and confocal laser scanning microscopy Digital CCD microscopy and image processing Each chapter includes practical demonstrations and exercises along with a discussion of the relevant material. In addition, a thorough glossary assists with complex terminology and an appendix contains lists of materials, procedures for specimen preparation, and answers to questions. An essential resource for both, experienced and novice microscopists.



Fundamentals of Light Microscopy and Electronic Imaging

Fundamentals of Light Microscopy and Electronic Imaging Author Douglas B. Murphy
ISBN-10 LCCN:2012009798
Release 2011
Pages 538
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Fundamentals of Light Microscopy and Electronic Imaging has been writing in one form or another for most of life. You can find so many inspiration from Fundamentals of Light Microscopy and Electronic Imaging also informative, and entertaining. Click DOWNLOAD or Read Online button to get full Fundamentals of Light Microscopy and Electronic Imaging book for free.



Video Microscopy

Video Microscopy Author Shinya Inoué
ISBN-10 0306455315
Release 1997-08-31
Pages 741
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Explains fundamentals of imaging and processing in the light microscope, the human visual system, video and related electronic imaging, and the digital image processor, and offers many how-to instructions for various procedures. Coverage includes practical aspects of microscopy, including selection,



Fundamentals of Fluorescence Microscopy

Fundamentals of Fluorescence Microscopy Author Partha Pratim Mondal
ISBN-10 9789400775459
Release 2013-12-12
Pages 218
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This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.



Introduction to Optical Microscopy

Introduction to Optical Microscopy Author Jerome Mertz
ISBN-10 0981519482
Release 2010
Pages 413
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Introduction to Optical Microscopy provides a rigorous and comprehensive overview of the fundamentals of optical microscopy. Starting from basic principles in Fourier optics, partial coherence, 3D imaging theory, and the physics of scattering and fluorescence, Introduction to Optical Microscopy explores a broad range of microscopy techniques. These include classical techniques such as phase contrast, confocal microscopy, etc., and progress to more modern techniques such as holographic microscopy, optical coherence tomography, two-photon microscopy, coherent anti-Stokes Raman scattering microscopy, etc.. The final chapters present a survey of new directions, including structured illumination and superresolution. Introduction to Optical Microscopy is designed to provide a solid theoretical foundation for graduate students or researchers who want to enter the field. While valuable as a reference, it can also serve as a textbook, as it includes a corresponding website that provides problem sets and an instructor's solution manual.



Light and Electron Microscopy

Light and Electron Microscopy Author Elizabeth M. Slayter
ISBN-10 0521339480
Release 1992-10-30
Pages 312
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This book describes the principles of operation of each type of microscope currently available and of use to biomedical and materials scientists. It explains the mechanisms of image formation, contrast and its enhancement, and accounts for ultimate limits on the size of observable details (resolving power and resolution). Finally it provides an account of Fourier optical theory. Principles behind the photographic methods used in microscopy are also described and there is some discussion of image processing methods. Throughout, the text emphasizes the underlying similarity of all microscope systems and, recognizing that biologists may often be uncomfortable with mathematical approaches every effort has been made to present concepts verbally.



Principles of Fluorescence Spectroscopy

Principles of Fluorescence Spectroscopy Author Joseph R. Lakowicz
ISBN-10 9781475730616
Release 2013-04-17
Pages 698
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`In the second edition of Principles I have attempted to maintain the emphasis on basics, while updating the examples to include more recent results from the literature. There is a new chapter providing an overview of extrinisic fluorophores. The discussion of timeresolved measurements has been expanded to two chapters. Quenching has also been expanded in two chapters. Energy transfer and anisotropy have each been expanded to three chapters. There is also a new chapter on fluorescence sensing. To enhance the usefulness of this book as a textbook, most chapters are followed by a set of problems. Sections which describe advanced topics are indicated as such, to allow these sections to be skipped in an introduction course. Glossaries are provided for commonly used acronyms and mathematical symbols. For those wanting additional informtion, the final appendix contains a list of recommended books which expand on various specialized topics.' from the author's Preface



Confocal Microscopy for Biologists

Confocal Microscopy for Biologists Author Alan R. Hibbs
ISBN-10 9780306485657
Release 2004-04-30
Pages 467
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There has been a great upsurge in interest in light microscopy in recent years due to the advent of a number of significant advances in microscopy, one of the most important of which is confocal microscopy. Confocal microscopy has now become an important research tool, with a large number of new fluorescent dyes becoming available in the past few years, for probing your pet structure or molecule within fixed or living cell or tissue sampies. Many of the people interested in using confocal microscopy to further their research do not have a background in microscopy or even cell biology and so not only do they find considerable difficulty in obtaining satisfactory results with a confocal microscope, but they may be mislead by how data is being presented. This book is intended to teach you the basic concepts ofmicroscopy, fluorescence, digital imaging and the principles of confocal microscopy so that you may take full advantage ofthe excellent confocal microscopes now available. This book is also an excellent reference source for information related to confocal microscopy for both beginners and the more advanced users. For example, do you need to know the optimal pinhole size for a 63x 1. 4 NA lens? Do you need to know the fluorescence emission spectrum of Alexa 568? Access to the wealth of practical information in this book is made easier by using both the detailed index and the extensive glossary.



Optical Imaging Techniques in Cell Biology Second Edition

Optical Imaging Techniques in Cell Biology  Second Edition Author Guy Cox
ISBN-10 9781439848258
Release 2012-06-04
Pages 316
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Optical Imaging Techniques in Cell Biology, Second Edition covers the field of biological microscopy, from the optics of the microscope to the latest advances in imaging below the traditional resolution limit. It includes the techniques—such as labeling by immunofluorescence and fluorescent proteins—which have revolutionized cell biology. Quantitative techniques such as lifetime imaging, ratiometric measurement, and photoconversion are all covered in detail. Expanded with a new chapter and 40 new figures, the second edition has been updated to cover the latest developments in optical imaging techniques. Explanations throughout are accurate, detailed, but as far as possible non-mathematical. This edition includes appendices with useful practical protocols, references, and suggestions for further reading. Color figures are integrated throughout.



Introduction to Light Microscopy

Introduction to Light Microscopy Author Savile Bradbury
ISBN-10 038791515X
Release 1998
Pages 123
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This book provides detailed and fully illustrated advice on choosing and using the appropriate type of light microscope for a particular application. The low-power stereomicroscope is described, and the many different types of condensers, objectives and eyepieces required for the high-power compound microscope are explained in detail. The book also describes the correct care and use of the microscope in order to achieve the best possible image, and provides a checklist to aid in the diagnosis and correction of problems. Practical step-by-step guidance ensures that the reader always obtains a clear image, Introduction to Light Microscopy is therefore an essential guide for amateur and professional users of the light microscope in all areas of science.



Digital Microscopy

Digital Microscopy Author Greenfield Sluder
ISBN-10 0080544347
Release 2007-04-26
Pages 632
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The previous edition of this book marked the shift in technology from video to digital camera use with microscope use in biological science. This new edition presents some of the optical fundamentals needed to provide a quality image to the digital camera. Specifically, it covers the fundamental geometric optics of finite- and infinity-corrected microscopes, develops the concepts of physical optics and Abbe’s theory of image formation, presents the principles of Kohler illumination, and finally reviews the fundamentals of fluorescence and fluorescence microscopy. The second group of chapters deals with digital and video fundamentals: how digital and video cameras work, how to coordinate cameras with microscopes, how to deal with digital data, the fundamentals of image processing, and low light level cameras. The third group of chapters address some specialized areas of microscopy that allow sophisticated measurements of events in living cells that are below the optical limits of resolution. * Expands coverage to include discussion of confocal microscopy not found in the previous edition * Includes "traps and pitfalls" as well as laboratory exercises to help illustrate methods



Super Resolution Microscopy

Super Resolution Microscopy Author Udo J. Birk
ISBN-10 9783527341337
Release 2017-12-04
Pages 408
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This unique book on super-resolution microscopy techniques presents comparative, in-depth analyses of the strengths and weaknesses of the individual approaches. It was written for non-experts who need to understand the principles of super-resolution or who wish to use recently commercialized instruments as well as for professionals who plan to realize novel microscopic devices. Explaining the practical requirements in terms of hardware, software and sample preparation, the book offers a wealth of hands-on tips and practical tricks to get a setup running, provides invaluable help and support for successful data acquisition and specific advice in the context of data analysis and visualization. Furthermore, it addresses a wide array of transdisciplinary fields of applications. The author begins by outlining the joint efforts that have led to achieving super-resolution microscopy combining advances in single-molecule photo-physics, fluorophore design and fluorescent labeling, instrument design and software development. The following chapters depict and compare current main standard techniques such as structured illumination microscopy, single-molecule localization, stimulated emission depletion microscopy and multi-scale imaging including light-sheet and expansion microscopy. For each individual approach the experimental setups are introduced, the imaging protocols are provided and the various applications illustrated. The book concludes with a discussion of future challenges addressing issues of routine applications and further commercialization of the available methods. Guiding users in how to make choices for the design of their own experiments from scratch to promising application, this one-stop resource is intended for researchers in the applied sciences, from chemistry to biology and medicine to physics and engineering.



Handbook of Biological Confocal Microscopy

Handbook of Biological Confocal Microscopy Author James Pawley
ISBN-10 9781461571339
Release 2012-12-06
Pages 232
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In 1987 the Electron Microscopy Society of America (EMSA) going to drive important scientific discoveries across wide areas under the leadership of J. P. Revel (Cal Tech) initiated a major of physiology, cellular biology and neurobiology. They had been program to present a discussion of recent advances in light looking for a forum in which they could advance the state of microscopy as part of the annual meeting. The result was three the art of confocal microscopy, alert manufacturers to the lim special LM sessions at the Milwaukee meeting in August 1988: itations of current instruments, and catalyze progress toward The LM Forum, organized by me, and Symposia on Confocal new directions in confocal instrument development. LM, organized by G. Schatten (Madison), and on Integrated These goals were so close to those of the EMSA project that Acoustic/LM/EM organized by C. Rieder (Albany). In addition, the two groups decided to join forces with EMSA to provide there was an optical micro-analysis session emphasizing Raman the organization and the venue for a Confocal Workshop and techniques, organized by the Microbeam Analysis Society, for NSF to provide the financial support for the speakers expenses a total of 40 invited and 30 contributed papers on optical tech and for the publication of extended abstracts.



Practical Digital Photomicrography

Practical Digital Photomicrography Author Brian Matsumoto
ISBN-10 1933952075
Release 2010
Pages 175
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Practical Digital Photomicrography is an introduction to digital photography through the microscope in which readers will learn about the techniques and equipment needed to obtain high-quality images of biological subjects. Author Dr. Brian Matsumoto received his Ph.D. in Anatomy and Cell Biology from the University of California at Los Angeles in 1981. He served as the Director of the Integrated Microscopy Facility in the Department of Molecular, Cellular, and Developmental Biology at the University of California, Santa Barbara from 1992 through 2009, where he taught a graduate level course in digital microscopy. Leveraging his decades of experience, Brian demystifies photomicrography basics such as microscope and camera concepts, illumination techniques, specimen preparation, photographic techniques, advanced optical techniques, and image post-processing. Abundant illustrations will guide the reader through these topics. Professionals in the life sciences and photography, educators and students, as well as photography enthusiasts will find this book an invaluable guide to learning the skills needed to obtain professional quality images through the microscope.



Physical Principles of Electron Microscopy

Physical Principles of Electron Microscopy Author Ray Egerton
ISBN-10 0387258000
Release 2011-02-11
Pages 202
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Scanning and stationary-beam electron microscopes are indispensable tools for both research and routine evaluation in materials science, the semiconductor industry, nanotechnology and the biological, forensic, and medical sciences. This book introduces current theory and practice of electron microscopy, primarily for undergraduates who need to understand how the principles of physics apply in an area of technology that has contributed greatly to our understanding of life processes and "inner space." Physical Principles of Electron Microscopy will appeal to technologists who use electron microscopes and to graduate students, university teachers and researchers who need a concise reference on the basic principles of microscopy.



Understanding Biophotonics

Understanding Biophotonics Author Kevin Tsia
ISBN-10 9789814411783
Release 2016-01-05
Pages 766
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Biophotonics involves understanding how light interacts with biological matter, from molecules and cells, to tissues and even whole organisms. Light can be used to probe biomolecular events, such as gene expression and protein–protein interaction, with impressively high sensitivity and specificity. The spatial and temporal distribution of biochemical constituents can also be visualized with light and, thus, the corresponding physiological dynamics in living cells, tissues, and organisms in real time. Light can also be used to alter the properties and behaviors of biological matter, such as to damage cancerous cells by laser surgery or therapy, and manipulate the neuronal signaling in a brain network. Fueled by the innovations in photonic technologies in the past half century, biophotonics continues to play a ubiquitous role in revolutionizing basic life science studies as well as biomedical diagnostics and therapies. Advancements in biophotonics in the past few decades can be seen not only in biochemistry and cell/molecular biology, but also in numerous preclinical applications. Researchers around the world are searching for ways to bring biophotonic technologies into real clinical practices, particularly cellular and molecular optical imaging. Meanwhile, emerging technologies, such as laser nanosurgery and nanoplasmonics, have created new insights for understanding, monitoring, and even curing diseases on a molecular basis. This book presents the essential basics of optics and biophotonics to newcomers (senior undergraduates or postgraduate researchers) who are interested in this multidisciplinary research field. With stellar contributions from leading experts, the book highlights the major advancements in preclinical diagnostics using optical microscopy and spectroscopy, including multiphoton microscopy, super-resolution microscopy, and endomicroscopy. It also introduces a number of emerging techniques and toolsets for biophotonics applications, such as nanoplasmonics, microresonators for molecular detection, and subcellular optical nanosurgery.