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PCR

PCR Author Mike McPherson
ISBN-10 9781134254569
Release 2007-01-24
Pages 292
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A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.



PCR

PCR Author Mike McPherson
ISBN-10 9780203002674
Release 2007-01-25
Pages 292
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A thoroughly updated version of the successful first edition with a new chapter on Real-Time PCR, more prokaryotic applications, and more detail in the complex mutagenesis sections. Information on PCR applications in genomics and proteomics have been expanded and integrated throughout the text. There is also advice on available products and specific pointers to the most appropriate methods. As with the first edition, this will be an ideal practical introduction and invaluable guide to PCR and its applications.



Understanding PCR

Understanding PCR Author Sarah Maddocks
ISBN-10 9780128026977
Release 2016-10-27
Pages 98
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Understanding PCR: A Practical Bench-Top Guide gives you all of the information you need to plan your first PCR, from reagents to conditions to analysis and beyond. It is a user friendly book that has step-by-step basic protocols, which can be adapted to your needs. Includes helpful information such as where to order your reagents and basic troubleshooting hints and tips. Includes resources for reagents Explains basic laboratory preparation Provides straightforward experimental protocols Incorporates fundamental analytical techniques Contains a troubleshooting guide



Real Time PCR

Real Time PCR Author M Tevfik Dorak
ISBN-10 9781134183999
Release 2007-01-24
Pages 333
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With a variety of detection chemistries, an increasing number of platforms, multiple choices for analytical methods and the jargon emerging along with these developments, real-time PCR is facing the risk of becoming an intimidating method, especially for beginners. Real-time PCR provides the basics, explains how they are exploited to run a real-time PCR assay, how the assays are run and where these assays are informative in real life. It addresses the most practical aspects of the techniques with the emphasis on 'how to do it in the laboratory'. Keeping with the spirit of the Advanced Methods Series, most chapters provide an experimental protocol as an example of a specific assay.



PCR Guru

PCR Guru Author Ayaz Najafov
ISBN-10 9780128042465
Release 2016-11-28
Pages 104
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PCR Guru: An Ultimate Benchtop Reference for Molecular Biologists is provides researchers in molecular biology with a handy reference for approaching and solving challenging problems associated with PCR setup and optimization. As a laboratory guide, it emphasizes the technical aspects of employing PCR as a tool in molecular biology laboratories. The book covers the history of PCR and the basic science underlying it. It then discusses PCR at the bench level, starting with detailed description and tips on primer design, and continuing with the standard protocols used to perform PCR. Provides troubleshooting tips for various types of modifications of standard protocols Contains unique “Good Practices and Tips that are indispensable for the beginner and expert alike Features “Special Cases with applications of PCR, optimization, and troubleshooting Includes detailed appendices with tables, figures, and key protocols Organized as a systematic, concentrated resource to save time when addressing a PCR problem



Quantitative Real Time PCR

Quantitative Real Time PCR Author Roberto Biassoni
ISBN-10 149394665X
Release 2016-08-23
Pages 231
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Quantitative Real-Time PCR: Methods and Protocols focuses on different applications of qPCR ranging from microbiological detections (both viral and bacterial) to pathological applications. Several chapters deal with quality issues which regard the quality of starting material, the knowledge of the minimal information required to both perform an assay and to set the experimental plan, while the others focus on translational medicine applications that are ordered following an approximate logical order of their medical application. The last part of the book gives you an idea of an emerging digital PCR technique that is a unique qPCR approach for measuring nucleic acid, particularly suited for low level detection and to develop non-invasive diagnosis. Written for the Methods in Molecular Biology series, most chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, laboratory protocols and tips on troubleshooting and avoiding known pitfalls. Practical and authoritative, Quantitative Real-Time PCR: Methods and Protocols aims to aid researchers seeking to devise new qPCR-based approaches related to his or her area of investigation.



Molecular Biology of the Cell

Molecular Biology of the Cell Author Bruce Alberts
ISBN-10 9781317563747
Release 2017-08-07
Pages 1464
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As the amount of information in biology expands dramatically, it becomes increasingly important for textbooks to distill the vast amount of scientific knowledge into concise principles and enduring concepts.As with previous editions, Molecular Biology of the Cell, Sixth Edition accomplishes this goal with clear writing and beautiful illustrations. The Sixth Edition has been extensively revised and updated with the latest research in the field of cell biology, and it provides an exceptional framework for teaching and learning. The entire illustration program has been greatly enhanced.Protein structures better illustrate structure–function relationships, icons are simpler and more consistent within and between chapters, and micrographs have been refreshed and updated with newer, clearer, or better images. As a new feature, each chapter now contains intriguing openended questions highlighting “What We Don’t Know,” introducing students to challenging areas of future research. Updated end-of-chapter problems reflect new research discussed in the text, and these problems have been expanded to all chapters by adding questions on developmental biology, tissues and stem cells, pathogens, and the immune system.



PCR Primer Design

PCR Primer Design Author Chhandak Basu
ISBN-10 1493947559
Release 2016-10-08
Pages 216
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This volume provides an overview on design PCR primers for successful DNA amplification. Chapters focus on primer design strategies for quantitative PCR, in silico PCR primer design, and primer design using software. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, PCR Primer Design, Second Edition seeks to aid molecular biology students, researchers, professors and PCR enthusiasts.



Forensic Science

Forensic Science Author Kathy Mirakovits
ISBN-10 9781439895054
Release 2016-04-19
Pages 525
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As forensic science continues to play a wider role in the investigation of crimes and apprehension of criminals, those without crime scene or crime lab training must now become familiar with the techniques and language of the forensic scientist. Avoiding the complicated science and graphic violence typical of most forensic references, this book is written specifically for those without forensic science experience. While it provides a professional reference for those not steeped in the details of forensic science, the wealth of instructor material available for teachers and its pedagogical approach make this an ideal textbook for high school and introductory level courses. Following up on the tremendously popular first edition, Forensic Science: The Basics, Second Edition now adds the insight of a new co-author who is known nationally for training instructors how to teach forensic science at all levels of education. The book takes readers from the initial evidence collection process, through the evaluation procedures, right up to and including the courtroom presentation. Packed with case studies, photographs, and exercises, this book provides everything the non-scientist needs to be able to understand and utilize the vital research approaches that forensic science can offer. "Test Yourself" questions at the end of each chapter familiarize you with the language and approaches needed to understand and communicate with experienced crime scene investigators and laboratory personnel. Offering the forensic sciences at their most accessible, Forensic Science: The Basics, Second Edition is a valuable resource for detectives, journalists, prosecutors, defense attorneys, and other non-science professionals who need to understand, interpret, and report on the newest advances in crime scene investigation. PowerPoint® lecture slides, test bank, and other ancillary material on CD-ROM is available with qualifying course adoption



Introducing Genetics

Introducing Genetics Author Alison Thomas
ISBN-10 9781317417262
Release 2014-12-18
Pages 270
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The new edition of Introducing Genetics is a clear, concise, and accessible guide to inheritance and variation in individuals and populations. It first establishes the principles of Mendelian inheritance and the nature of chromosomes, before tackling quantitative and population genetics. The final three chapters introduce the molecular mechanisms that underlie genetics, including the techniques responsible for the current genetic revolution. As genetics affects all living organisms, examples are taken from insects, plants, animals, and humans. Each chapter ends with a set of problems to help the reader develop their understanding of genetics.



Peptide Nucleic Acids

Peptide Nucleic Acids Author Peter E. Nielsen
ISBN-10 0954523245
Release 2004
Pages 318
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Peptide Nucleic Acids, Second Edition has been extensively revised, updated, and enlarged to contain many new chapters covering the most recent topics and applications in this fast-moving field. The book contains state-of-the-art protocols and applications on all aspects of peptide nucleic acids. Concepts are clearly explained with each chapter containing concise background information. Written by leading experts in the field, the book is an invaluable and complete reference work on this novel and exciting area.



Genomes 3

Genomes 3 Author Terence A. Brown
ISBN-10 9780815341383
Release 2007
Pages 713
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The VitalBook e-book version of Genomes 3 is only available in the US and Canada at the present time. To purchase or rent please visit http://store.vitalsource.com/show/9780815341383 Covering molecular genetics from the basics through to genome expression and molecular phylogenetics, Genomes 3is the latest edition of this pioneering textbook. Updated to incorporate the recent major advances, Genomes 3 is an invaluable companion for any undergraduate throughout their studies in molecular genetics. Genomes 3 builds on the achievements of the previous two editions by putting genomes, rather than genes, at the centre of molecular genetics teaching. Recognizing that molecular biology research was being driven more by genome sequencing and functional analysis than by research into genes, this approach has gathered momentum in recent years.



PCR Primer Design

PCR Primer Design Author Anton Yuryev
ISBN-10 9781597455282
Release 2007
Pages 431
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In the past decade, molecular biology has been transformed from the art of cloning a single gene to a statistical science measuring and calculating properties of entire genomes. New high-throughput methods have been developed for genome sequencing and studying the cell at different systematic levels such as transcriptome, proteome, metabolome and other -omes. At the heart of most high-throughput methods is the technique of polymerase chain reaction (PCR). PCR Primer Design focuses on primer design, which is critical to both the efficiency and the accuracy of the PCR. With intricate descriptions of basic approaches as well as specialized methods, this volume is an exceptional reference for all those involved in studying the genome. In PCR Primer Design, authors describe basic approaches for PCR primer design in addition to specialized methods. These state-of-the-art methods can be used for both genome-scale experiments and for small-scale individual PCR amplifications. This volume will be useful for organizations performing whole genome studies, companies designing instruments that utilize PCR, and individual scientists ??? geneticists, molecular biologists, molecular geneticists, and more ??? who routinely use PCR in their research.



Living in a Microbial World Second Edition

Living in a Microbial World  Second Edition Author Bruce Hofkin
ISBN-10 9781315294001
Release 2017-03-02
Pages 496
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As with the first edition, this new edition of Living In A Microbial World is written for students taking a general microbiology course, or a microbiology-based course for non-science majors. The conversational style and use of practical, everyday examples make the essential concepts of microbiology accessible to a wide audience- While using this approach, the text maintains scientific rigour with clear explanations spanning the breadth of microbiology, including health, evolution, ecology, food production, biotechnology, and industrial processes- Each chapter contains a series of case studies based on microbiology in the news, in history, and in literature- There are questions at the end of each case study and the end of each chapter, as well as an online quiz with help on answering the questions- The text, questions, and cases have been updated to reflect the changing influence of microbiology in the world today, from the microbiome, to new disease outbreaks (Ebola and Zika) and antibiotic resistance, to new biotechnology tools (CRISPR-Cas).



The PCR Revolution

The PCR Revolution Author Stephen A. Bustin
ISBN-10 9780521882316
Release 2010
Pages 307
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Examines the latest innovations and the overall impact of PCR on areas of molecular research.



Human Molecular Genetics

Human Molecular Genetics Author Tom Strachan
ISBN-10 9781136844072
Release 2018-03-29
Pages 782
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Human Molecular Genetics is an established and class-proven textbook for upper-level undergraduates and graduate students which provides an authoritative and integrated approach to the molecular aspects of human genetics. While maintaining the hallmark features of previous editions, the Fourth Edition has been completely updated. It includes new Key Concepts at the beginning of each chapter and annotated further reading at the conclusion of each chapter, to help readers navigate the wealth of information in this subject. The text has been restructured so genomic technologies are integrated throughout, and next generation sequencing is included. Genetic testing, screening, approaches to therapy, personalized medicine, and disease models have been brought together in one section. Coverage of cell biology including stem cells and cell therapy, studying gene function and structure, comparative genomics, model organisms, noncoding RNAs and their functions, and epigenetics have all been expanded.



A Low Cost Approach to PCR

A Low Cost Approach to PCR Author Eva Harris
ISBN-10 9780195119268
Release 1998-12-31
Pages 304
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The polymerase chain reaction (PCR) is a technique used to replicate specific pieces of DNA millions of times, which permits the detection and analysis of minute amounts of nucleic acids. Since its introduction in the late 1980s, this technique has been applied not only in molecular biology research but also in fields as diverse as anthropology, phylogeny, and forensics. However, despite the large impact of PCR, many of its applications remain within the confines of research and the academic environment. Now, in A Low-Cost Approach to PCR: Appropriate Transfer of Biomolecular Techniques, Dr. Eva Harris makes this elegantly simple technique more accessible to researchers, physicians, and laboratory workers throughout the world. She provides a description of the theoretical basis of the technique, the practical details of the method, and the philosophy behind the technology transfer program that she developed over the last ten years. The book serves as a guide for potential users in developing countries and for scientists in developed countries who may wish to work abroad. In addition, the low-cost approach outlined in this book can be useful for high school, undergraduate, or continuing education programs in the United States. While the specific applications of PCR outlined in the book are immediately useful to the study of infectious diseases, the approach presented can be generalized to a number of other technologies and situations. The book will help laboratories in many areas of the world generate information on site for use by physicians, epidemiologists, public health workers, and health policy professionals to develop new strategies for disease control.