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Quantitative Phase Imaging of Cells and Tissues

Quantitative Phase Imaging of Cells and Tissues Author Gabriel Popescu
ISBN-10 9780071663434
Release 2011-03-14
Pages 400
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Cutting-edge quantitative phase imaging techniques and their applications Filled with unique, full-color images taken by advanced quantitative phase imaging (QPI), Quantitative Phase Imaging of Cells and Tissues thoroughly explores this innovative technology and its biomedical applications. An introductory background on optical imaging and traditional optical microscopy is included to illustrate concept development. The book explains how various visualization modalities can be obtained by numerical calculations. This authoritative resource reveals how to take full advantage of the unprecedented capabilities of QPI, such as rendering scattering properties of minute subcellular structures and nanoscale fluctuations in live cells. Coverage includes: Groundwork Spatiotemporal field correlations Image characteristics Light microscopy Holography Point scanning QPI methods Principles of full-field QPI Off-axis full-field methods Phase-shifting techniques Common-path methods White light techniques Fourier transform light scattering (FTLS) Current trends in QPI



Introduction to Optical Microscopy

Introduction to Optical Microscopy Author Jerome Mertz
ISBN-10 0981519482
Release 2010
Pages 413
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Introduction to Optical Microscopy provides a rigorous and comprehensive overview of the fundamentals of optical microscopy. Starting from basic principles in Fourier optics, partial coherence, 3D imaging theory, and the physics of scattering and fluorescence, Introduction to Optical Microscopy explores a broad range of microscopy techniques. These include classical techniques such as phase contrast, confocal microscopy, etc., and progress to more modern techniques such as holographic microscopy, optical coherence tomography, two-photon microscopy, coherent anti-Stokes Raman scattering microscopy, etc.. The final chapters present a survey of new directions, including structured illumination and superresolution. Introduction to Optical Microscopy is designed to provide a solid theoretical foundation for graduate students or researchers who want to enter the field. While valuable as a reference, it can also serve as a textbook, as it includes a corresponding website that provides problem sets and an instructor's solution manual.



Understanding Biophotonics

Understanding Biophotonics Author Kevin Tsia
ISBN-10 9789814411783
Release 2016-01-05
Pages 766
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Biophotonics involves understanding how light interacts with biological matter, from molecules and cells, to tissues and even whole organisms. Light can be used to probe biomolecular events, such as gene expression and protein–protein interaction, with impressively high sensitivity and specificity. The spatial and temporal distribution of biochemical constituents can also be visualized with light and, thus, the corresponding physiological dynamics in living cells, tissues, and organisms in real time. Light can also be used to alter the properties and behaviors of biological matter, such as to damage cancerous cells by laser surgery or therapy, and manipulate the neuronal signaling in a brain network. Fueled by the innovations in photonic technologies in the past half century, biophotonics continues to play a ubiquitous role in revolutionizing basic life science studies as well as biomedical diagnostics and therapies. Advancements in biophotonics in the past few decades can be seen not only in biochemistry and cell/molecular biology, but also in numerous preclinical applications. Researchers around the world are searching for ways to bring biophotonic technologies into real clinical practices, particularly cellular and molecular optical imaging. Meanwhile, emerging technologies, such as laser nanosurgery and nanoplasmonics, have created new insights for understanding, monitoring, and even curing diseases on a molecular basis. This book presents the essential basics of optics and biophotonics to newcomers (senior undergraduates or postgraduate researchers) who are interested in this multidisciplinary research field. With stellar contributions from leading experts, the book highlights the major advancements in preclinical diagnostics using optical microscopy and spectroscopy, including multiphoton microscopy, super-resolution microscopy, and endomicroscopy. It also introduces a number of emerging techniques and toolsets for biophotonics applications, such as nanoplasmonics, microresonators for molecular detection, and subcellular optical nanosurgery.



Coherent Domain Optical Methods

Coherent Domain Optical Methods Author Valery V. Tuchin
ISBN-10 1402075766
Release 2004-10-15
Pages 1004
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For the first time in one set of books, coherent-domain optical methods are discussed in the framework of various applications, which are characterized by a strong light scattering. A few chapters describe basic research containing the updated results on coherent and polarized light non-destructive interactions with a scattering medium, in particular, diffraction, interference, and speckle formation at multiple scattering. These chapters allow for understanding coherent-domain diagnostic techniques presented in later chapters. A large portion of Volume I is dedicated to analysis of various aspects of optical coherence tomography (OCT) - a very new and growing field of coherent optics. Two chapters on laser scanning confocal microscopy give insight to recent extraordinary results on in vivo imaging and compare the possibilities and achievements of confocol, excitation multiphoton, and OCT microscopy. This two volume reference contains descriptions of holography, interferometry and optical heterodyning techniques in their application for diagnostics of turbid materials. The most prospective methods of coherent and polarization optical imaging and spectroscopy, including polarization-sensitive optical coherent tomography, polarization diffusion wave spectroscopy, and elastic and quasi-elastic light scattering spectroscopies and image techniques, are presented.



Introduction to Biophotonics

Introduction to Biophotonics Author Paras N. Prasad
ISBN-10 9780471465393
Release 2004-01-16
Pages 593
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Paras Prasad’s text provides a basic knowledge of a broad range of topics so that individuals in all disciplines can rapidly acquire the minimal necessary background for research and development in biophotonics. Introduction to Biophotonics serves as both a textbook for education and training as well as a reference book that aids research and development of those areas integrating light, photonics, and biological systems. Each chapter contains a topic introduction, a review of key data, and description of future directions for technical innovation. Introduction to Biophotonics covers the basic principles of Optics Optical spectroscopy Microscopy Each section also includes illustrated examples and review questions to test and advance the reader’s knowledge. Sections on biosensors and chemosensors, important tools for combating biological and chemical terrorism, will be of particular interest to professionals in toxicology and other environmental disciplines. Introduction to Biophotonics proves a valuable reference for graduate students and researchers in engineering, chemistry, and the life sciences.



Biomedical Optical Phase Microscopy and Nanoscopy

Biomedical Optical Phase Microscopy and Nanoscopy Author Natan T. Shaked
ISBN-10 9780124158719
Release 2012-11-05
Pages 411
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Written by leading optical phase microscopy experts, this book is a comprehensive reference to phase microscopy and nanoscopy techniques for biomedical applications, including differential interference contrast (DIC) microscopy, phase contrast microscopy, digital holographic microscopy, optical coherence tomography, tomographic phase microscopy, spectral-domain phase detection, and nanoparticle usage for phase nanoscopy The Editors show biomedical and optical engineers how to use phase microscopy for visualizing unstained specimens, and support the theoretical coverage with applied content and examples on designing systems and interpreting results in bio- and nanoscience applications. Provides a comprehensive overview of the principles and techniques of optical phase microscopy and nanoscopy with biomedical applications. Tips/advice on building systems and working with advanced imaging biomedical techniques, including interpretation of phase images, and techniques for quantitative analysis based on phase microscopy. Interdisciplinary approach that combines optical engineering, nanotechnology, biology and medical aspects of this topic. Each chapter includes practical implementations and worked examples.



Nanophysics Nanomaterials Interface Studies and Applications

Nanophysics  Nanomaterials  Interface Studies  and Applications Author Olena Fesenko
ISBN-10 9783319564227
Release 2017-09-04
Pages 880
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This book presents some of the latest achievements in nanotechnology and nanomaterials from leading researchers in Ukraine, Europe, and beyond. It features selected peer-reviewed contributions from participants in the 4th International Science and Practice Conference Nanotechnology and Nanomaterials (NANO2016) held in Lviv, Ukraine on August 24-27, 2016. The International Conference was organized jointly by the Institute of Physics of the National Academy of Sciences of Ukraine, Ivan Franko National University of Lviv (Ukraine), University of Tartu (Estonia), University of Turin (Italy), and Pierre and Marie Curie University (France). Internationally recognized experts from a wide range of universities and research institutions share their knowledge and key results on topics ranging from nanooptics, nanoplasmonics, and interface studies to energy storage and biomedical applications.



Biomedical Applications of Light Scattering

Biomedical Applications of Light Scattering Author Adam Wax
ISBN-10 9780071598811
Release 2009-09-22
Pages 350
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Clinical applications include: detecting pre-cancerous and cancerous tissue states; characterizing cell and tissue properties for identifying disease; and assessing the presence and concentration of biochemicals for diagnostic purposes Part of the McGraw-Hill Biophotonics Series



Integrative Biophysics

Integrative Biophysics Author Fritz-Albert Popp
ISBN-10 9789401703734
Release 2013-03-09
Pages 504
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Most of the specialists working in this interdisciplinary field of physics, biology, biophysics and medicine are associated with "The International Institute of Biophysics" (IIB), in Neuss, Germany, where basic research and possibilities for applications are coordinated. The growth in this field is indicated by the increase in financial support, interest from the scientific community and frequency of publications. Audience: The scientists of IIB have presented the most essential background and applications of biophotonics in these lecture notes in biophysics, based on the summer school lectures by this group. This book is devoted to questions of elementary biophysics, as well as current developments and applications. It will be of interest to graduate and postgraduate students, life scientists, and the responsible officials of industries and governments looking for non-invasive methods of investigating biological tissues.



Micro and Nanophotonic Technologies

Micro  and Nanophotonic Technologies Author Patrick Meyrueis
ISBN-10 9783527699933
Release 2017-03-20
Pages 571
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Edited and authored by leading experts from top institutions in Europe, the US and Asia, this comprehensive overview of micro- and nanophotonics covers the physical and chemical fundamentals, while clearly focusing on the technologies and applications in industrial R&D. As such, the book reports on the four main areas of telecommunications and display technologies; light conversion and energy generation; light-based fabrication of materials; and micro- and nanophotonic devices in metrology and control.



Fundamentals and Applications of Biophotonics in Dentistry

Fundamentals and Applications of Biophotonics in Dentistry Author Anil Kishen
ISBN-10 9781908979469
Release 2006-12-18
Pages 340
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Biophotonics in dentistry is a rapidly growing area. Unlike other books, this invaluable compendium touches on the fundamental areas in biophotonics. Contributed by world-renowned authors, it provides a basic understanding on a range of topics for individuals of different backgrounds to acquire a minimum knowledge of research and development in biophotonics. The chapters are arranged in two major categories. The first describes the fundamental aspects of photonics, such as photomechanics, biomedical imaging, lasers and laser-tissue interaction, spectroscopy and photodynamic therapy. The second details the applications of biophotonics, with special relevance to dentistry, including dental photobiomechanics, Raman spectroscopy and dental tissue optics. Contents:PhotomechanicsBiomedical ImagingSpectroscopyLasers and Laser Tissue InteractionMechanisms and Applications of Photodynamic TherapyDental Photo-BiomechanicsMicro-Raman Spectroscopy: Principles and Applications in Dental ResearchDental and Oral Tissue OpticsFiber Optic Diagnostic Sensors Readership: Researchers, academics and graduate students of biophotonics in dentistry. Keywords:Biophotonics;Photomechanics;Bioimaging;Spectroscopy;DentistryKey Features:A comprehensive textbook ideal for a course on photonics in dentistryProvides an in-depth introduction to light-tissue interactions



Raman Spectroscopy for Nanomaterials Characterization

Raman Spectroscopy for Nanomaterials Characterization Author Challa S.S.R. Kumar
ISBN-10 9783642206207
Release 2012-03-30
Pages 645
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First volume of a 40-volume series on nanoscience and nanotechnology, edited by the renowned scientist Challa S.S.R. Kumar. This handbook gives a comprehensive overview about Raman spectroscopy for the characterization of nanomaterials. Modern applications and state-of-the-art techniques are covered and make this volume essential reading for research scientists in academia and industry.



Membrane Potential Imaging in the Nervous System and Heart

Membrane Potential Imaging in the Nervous System and Heart Author Marco Canepari
ISBN-10 9783319176413
Release 2015-08-03
Pages 516
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This volume discusses membrane potential imaging in the nervous system and in the heart and modern optical recording technology. Additionally, it covers organic and genetically-encoded voltage-sensitive dyes; membrane potential imaging from individual neurons, brain slices, and brains in vivo; optical imaging of cardiac tissue and arrhythmias; bio-photonics modelling. This is an expanded and fully-updated second edition, reflecting all the recent advances in this field. Twenty chapters, all authored by leading names in the field, are cohesively structured into four sections. The opening section focuses on the history and principles of membrane potential imaging and lends context to the following sections, which examine applications in single neurons, networks, large neuronal populations and the heart. Topics discussed include population membrane potential signals in development of the vertebrate nervous system, use of membrane potential imaging from dendrites and axons, and depth-resolved optical imaging of cardiac activation and repolarization. The final section discusses the potential – and limitations – for new developments in the field, including new technology such as non-linear optics, advanced microscope designs and genetically encoded voltage sensors. Membrane Potential Imaging in the Nervous System and Heart is ideal for neurologists, electro physiologists, cardiologists and those who are interested in the applications and the future of membrane potential imaging.



Fundamental Concepts in Biophysics

Fundamental Concepts in Biophysics Author Thomas Jue
ISBN-10 9781597453974
Release 2009-04-20
Pages 248
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In the first volume, Fundamental Concepts in Biophysics, the authors lay down a foundation for biophysics study. Rajiv Singh opens the book by pointing to the central importance of “Mathematical Methods in Biophysics”. William Fink follows with a discussion on “Quantum Mechanics Basic to Biophysical Methods”. Together, these two chapters establish some of the principles of mathematical physics underlying many biophysics techniques. Because computer modeling forms an intricate part of biophysics research, Subhadip Raychaudhuri and colleagues introduce the use of computer modeling in “Computational Modeling of Receptor–Ligand Binding and Cellular Signaling Processes”. Yin Yeh and coworkers bring to the reader’s attention the physical basis underlying the common use of fluorescence spectroscopy in biomedical research in their chapter “Fluorescence Spectroscopy”. Electrophysiologists have also applied biophysics techniques in the study of membrane proteins, and Tsung-Yu Chen et al. explore stochastic processes of ion transport in their “Electrophysiological Measurements of Membrane Proteins”. Michael Saxton takes up a key biophysics question about particle distribution and behavior in systems with spatial or temporal inhomogeneity in his chapter “Single–Particle Tracking”. Finally, in “NMR Measurement of Biomolecule Diffusion”, Thomas Jue explains how magnetic resonance techniques can map biomolecule diffusion in the cell to a theory of respiratory control. This book thus launches the Handbook of Modern Biophysics series and sets up for the reader some of the fundamental concepts underpinning the biophysics issues to be presented in future volumes.



Imaging Flow Cytometry

Imaging Flow Cytometry Author Natasha S. Barteneva
ISBN-10 1493933000
Release 2015-11-23
Pages 295
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This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.



Nanobiophotonics

Nanobiophotonics Author Gabriel Popescu
ISBN-10 9780071737029
Release 2010-07-23
Pages 432
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Cutting-edge coverage of nanobiophotonics Written by global experts, Nanobiophotonics offers in-depth details on this emerging, cross-disciplinary field. After discussions on cancer cell biology, electromagnetic fields, and nanophotonics, the book delves into nanobiophtoonics technologies and current research areas. This pioneering work covers the latest nanoscale and superresolution medical imaging methods and biomedical applications. Nanobiophotonics covers: Tissue pathology Light scattering by inhomogeneous media Nonlinear optics Vision restoration Optical low-coherence interferometric techniques Plasmonics and metamaterials Infrared spectroscopic imaging Scattering, absorbing, and modulating nanoprobes for coherence imaging Second-harmonic generation imaging of collagen-based systems Plasmon resonance energy transfer nanospectroscopy Erythrocyte nanoscale flickering as a marker for disease Superresolution far-field fluorescence microscopy



A Guided Tour of Light Beams

A Guided Tour of Light Beams Author David S Simon
ISBN-10 9781681744384
Release 2016-12-07
Pages 105
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From science fiction death rays to supermarket scanners, lasers have become deeply embedded in our daily lives and our culture. But in recent decades the standard laser beam has evolved into an array of more specialized light beams with a variety of strange and counterintuitive properties. Some of them have the ability to reconstruct themselves after disruption by an obstacle, while others can bend in complicated shapes or rotate like a corkscrew. These unusual optical effects open new and exciting possibilities for science and technology. For example, they make possible microscopic tractor beams that pull objects toward the source of the light, and they allow the trapping and manipulation of individual molecules to construct specially-tailored nanostructures for engineering or medical use. It has even been found that beams of light can produce lines of darkness that can be tied in knots. This book is an introductory survey of these specialized light beams and their scientific applications, at a level suitable for undergraduates with a basic knowledge of optics and quantum mechanics. It provides a unified treatment of the subject, collecting together in textbook form for the first time many topics currently found only in the original research literature.