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Tetrahymena Thermophila

Tetrahymena Thermophila Author
ISBN-10 0080524958
Release 1999-09-08
Pages 588
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Tetrahymena thermophila is emerging as a powerful experimental system for functional genetics. The ciliated protozoan offers numerous advantages, not the least of which is the ability to eliminate any specific gene of interest and then to evaluate the effect of that mutation on the living cell. Past investigations with T. thermophila have yielded several key discoveries, including dynein, catalytic RNA, and telomerase. This volume is a comprehensive resource for using Tetrahymena in the laboratory and is aimed at persons already experienced, as well as newcomers to the organism. It covers both the biological background and essential protocols for investigators rapidly turning to Tetrahymena as the experimental system of choice. Contains both theoretical and practical issues in 30 chapters contributed by the world authorities on Tetrahymena Indispensible for both the novice and the experienced researcher Overviews the history, cell biology, and genetics of the organism Describes essential protocols on the growth of cells, genetic techniques, and how to look at the cells with the microscope Illustrates how the methods can be applied to solve various cell biological problems Reviews recently developed strategies for altering gene expression



Tetrahymena Thermophila

Tetrahymena Thermophila Author
ISBN-10 9780123859686
Release 2012-10-22
Pages 430
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This new volume of Methods in Cell Biology looks at Tetrahymena thermophila, a model organism in experimental biology. Covering sections on Systems perspectives and Operating principles, chapters are written by experts in the field. With cutting edge material, this comprehensive collection is intended to guide researchers of Tetrahymena thermophila for years to come. Covers sections on systems perspectives and operating principles Chapters are written by experts in the field Cutting-edge material, making this a truly comprehensive collection



Cilia Motors and Regulation

Cilia  Motors and Regulation Author
ISBN-10 008096107X
Release 2009-12-01
Pages 400
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Along with its companion volume on axonemal dynein-mediated motility, this book provides researchers with a comprehensive and up-to-date source of methods for the analysis cilia and flagella, focusing primarily on approaches that have been devised or significantly extended since the last volume of Methods in Cell Biology on this topic (volume 47, 1995). Edited by Stephen M. King and Gregory J. Pazour, the newest installment of this highly acclaimed serial will serve as an essential addition to the study of cilia and flagella. * Covers protocols for cilia and flagella across systems and species * Both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Relevant to clinicians interested in respiratory disease, male infertility, and other syndromes who need to learn biochemical, molecular, and genetic approaches to studying cilia, flagella, and related structures



Cilia Model Organisms and Intraflagellar Transport

Cilia  Model Organisms and Intraflagellar Transport Author
ISBN-10 0123813786
Release 2009-12-01
Pages 416
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Cilia are highly conserved organelles that serve motile functions, sensory functions, or both. These organelles power cell movement, generate fluid flow in various organs, act as sensors of the extracellular environment and have been modified for various specialized tasks such as light reception and smell. Defects in these ubiquitous organelles lead to a broad array of human genetic disorders that range from polycystic kidney disease, retinal degeneration, epilepsy and infertility to developmental defects such as situs inversus and polydactyly. This volume is the third in a three-part series on cilia that focuses on the use of model organisms to gain insight into ciliary function and on the process of intraflagellar transport that is essential for the assembly and maintenance of ciliary structures. * Includes both classic and state-of-the-art methods readily adaptable across model systems, and designed to last the test of time * Covers forward and reverse genetic analysis of IFT and biochemical methods to define the role of IFT components * Methods presented cover molecular, genetic, and biochemical approaches to ciliary function in model organisms



Electron Microscopy of Model Systems

Electron Microscopy of Model Systems Author
ISBN-10 0123810086
Release 2010-09-24
Pages 744
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The volume covers the preparation and analysis of model systems for biological electron microscopy. The volume has chapters about prokaryotic as well as eukaryotic systems that are used as so-called model organisms in modern cell biology. These systems include the most popular systems, such as budding and fission yeast, the roundworm C. elegans, the fly Drosophila, zebrafish, mouse, and Arabidopsis, but also organisms that are less frequently used in cell biology, such as Chlamydomonas, Dictyostelium, Trypanosoma, faltworms, Axolotl and others. In addition, tissues and tissue culture systems are also covered. These systems are used for very diverse areas of cell biology, such as cell division, abscission, intracellular transport, cytoskeletal organization, tissue regeneration and others. Moreover, this issue presents the currently most important methods for the preparation of biological specimens. This volume, however, is not a classic EM methods book. The methods are not the main focus of this issue. The main goal here is to cover the methods in the context of the specific requirements of specimen preparation for each model organism or systems. This will be the first compendium covering the various aspects of sample preparation of very diverse biological systems. Covers the preparation and analysis of model systems for biological electron microscopy Includes the most popular systems but also organisms that are less frequently used in cell biology Presents the currently most important methods for the preparation of biological specimens First compendium covering the various aspects of sample preparation of very diverse biological systems



Tetrahymena Thermophila

Tetrahymena Thermophila Author Kathleen Collins
ISBN-10 9780123859679
Release 2012
Pages 452
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This new volume of Methods in Cell Biology looks at Tetrahymena thermophila, a model organism in experimental biology. Covering sections on Systems perspectives and Operating principles, chapters are written by experts in the field. With cutting edge material, this comprehensive collection is intended to guide researchers of Tetrahymena thermophila for years to come. Covers sections on systems perspectives and operating principles Chapters are written by experts in the field Cutting-edge material, making this a truly comprehensive collection



Cytoskeleton methods and protocols

Cytoskeleton methods and protocols Author Ray H. Gavin
ISBN-10 UOM:39015075686454
Release 2009-10-01
Pages 390
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In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding of the cytoskeleton as a dynamic synergy of components. In Cytoskeleton Methods and Protocols, Second Edition, internationally renowned experts present techniques which reflect many of the recent technological advances in experimental tools for cytoskeleton research with emphasis on animal, plant, protist, and fungal model systems. This cutting-edge volume contains methods for live-cell imaging, fluorescence microscopy, electron microscopy, analysis of cell and organelle motility, isolation of cytoskeleton components, and proteomics, amongst other topics. As a volume in the highly successful Methods in Molecular Biology'¢ series, chapters incorporate introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes that provide unpublished technical information on troubleshooting and avoiding known pitfalls.Up-to-date and comprehensive, Cytoskeleton Methods and Protocols, Second Edition serves as an ideal guide to scientists who wish to continue this fruitful and important biological research.



Trafficking Inside Cells

Trafficking Inside Cells Author Nava Segev
ISBN-10 9780387938776
Release 2010-05-30
Pages 445
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This book covers the past, present and future of the intra-cellular trafficking field, which has made a quantum leap in the last few decades. It details how the field has developed and evolved as well as examines future directions.



Cumulated Index Medicus

Cumulated Index Medicus Author
ISBN-10 UIUC:30112048191891
Release 2000
Pages
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Cumulated Index Medicus has been writing in one form or another for most of life. You can find so many inspiration from Cumulated Index Medicus also informative, and entertaining. Click DOWNLOAD or Read Online button to get full Cumulated Index Medicus book for free.



Molecular Biology of the Cell

Molecular Biology of the Cell Author
ISBN-10 STANFORD:36105122112191
Release 2006
Pages
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Molecular Biology of the Cell has been writing in one form or another for most of life. You can find so many inspiration from Molecular Biology of the Cell also informative, and entertaining. Click DOWNLOAD or Read Online button to get full Molecular Biology of the Cell book for free.



Protist Diversity and Geographical Distribution

Protist Diversity and Geographical Distribution Author W. Foissner
ISBN-10 9789048128013
Release 2009-07-24
Pages 211
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Conservation and biodiversity of protists The conservation of biodiversity is not just an issue of plants and vertebrates. It is the scarcely visible invertebrates and myriads of other microscopic organisms that are crucial to the maintenance of ecological processes on which all larger organisms and the composition of the atmosphere ultimately depend. Biodiversity and Conservation endeavours to take an holistic view of biodiversity, and when the opportunity arises to issue collections of papers dealing with too-often neglected groups of organisms. The protists, essentially eukaryotes that cannot be classi?ed in the kingdoms of animals, fungi, or plants, include some of the lea- known groups of organisms on earth. They are generally treated as a separate kingdom, commonly named Protista (or Protoctista) in textbooks, but in reality they are a mixture of organisms with disparate a?nities. Some authors have hypothesized that the numbers of protists are not especially large, and that many have extraordinarily wide distributions. However, the p- ture that unfolds from the latest studies discussed in this issue is di?erent. There are many species with wide ranges, and proportionately more cosmopolitan species than in macroorganism groups, as a result of their long evolutionary histories, but there are also de?nite patterns and geographical restrictions to be found. Further, some protists are linked to host organisms as mutualists or parasites and necessarily con?ned to the distributions of their hosts.



Of Urfs and Orfs

Of Urfs and Orfs Author Russell F. Doolittle
ISBN-10 0935702547
Release 1986
Pages 103
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In these days of facile cloning and rapid DNA sequencing, it is not uncommon for investigators to find themselves with a DNA sequence that may or may not code for a known gene product. The sequence is 'open' when read in an appropriate frame, which is to say that there is a long run of amino acid codons before the appearance of a terminator codon. How can we find out if this 'unidentified reading frame' (URF) really codes for a genuine protein, and how can we identify it if it exists?



RNA Infrastructure and Networks

RNA Infrastructure and Networks Author Lesley J. Collins
ISBN-10 9781461403326
Release 2011-09-15
Pages 280
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RNAs form complexes with proteins and other RNAs. The RNA‐infrastructure represents the spatiotemporal interaction of these proteins and RNAs in a cell‐wide network. RNA Infrastructure and Networks brings together these ideas to illustrate the scope of RNA‐based biology, and how connecting RNA mechanisms is a powerful tool to investigate regulatory pathways. This book is but a taste of the wide range of RNA‐based mechanisms that connect in the RNA infrastructure.



Methods in Cell Biology

Methods in Cell Biology Author
ISBN-10 0080858988
Release 1964-01-01
Pages 464
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Methods in Cell Biology



Small Noncoding RNAs and Their Biogenesis in Tetrahymena Thermophila

Small Noncoding RNAs and Their Biogenesis in Tetrahymena Thermophila Author Suzanne Rebecca Lee
ISBN-10 UCAL:C3511515
Release 2007
Pages 240
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Small Noncoding RNAs and Their Biogenesis in Tetrahymena Thermophila has been writing in one form or another for most of life. You can find so many inspiration from Small Noncoding RNAs and Their Biogenesis in Tetrahymena Thermophila also informative, and entertaining. Click DOWNLOAD or Read Online button to get full Small Noncoding RNAs and Their Biogenesis in Tetrahymena Thermophila book for free.



The Yeasts

The Yeasts Author Cletus Kurtzman
ISBN-10 0080931278
Release 2011-05-09
Pages 2354
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The Yeasts: A Taxonomic Study is a three-volume book that covers the taxonomic aspect of yeasts. The main goal of this book is to provide important information about the identification of yeasts. It also discusses the growth tests that can be used to identify different species of yeasts, and it examines how the more important species of yeasts provide information for the selection of species needed for biotechnology. • Volume 1 discusses the identification, classification and importance of yeasts in the field of biotechnology. • Volume 2 focuses on the identification and classification of ascomycetous yeasts. • Volume 3 deals with the identification and classification of basidiomycetous yeasts, along with the genus Prototheca. High-quality photomicrographs and line drawings Detailed phylogenetic trees Up-to-date, clearly presented yeast taxonomy and systematic, easy-to-use reference sequence accession numbers to allow for correct identification



Introduction to Evolutionary Genomics

Introduction to Evolutionary Genomics Author Naruya Saitou
ISBN-10 9781447153047
Release 2014-01-22
Pages 461
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This book is the first of its kind to explain the fundamentals of evolutionary genomics. The comprehensive coverage includes concise descriptions of a variety of genome organizations, a thorough discussion of the methods used, and a detailed review of genome sequence processing procedures. The opening chapters also provide the necessary basics for readers unfamiliar with evolutionary studies. Features: introduces the basics of molecular biology, DNA replication, mutation, phylogeny, neutral evolution, and natural selection; presents a brief evolutionary history of life from the primordial seas to the emergence of humans; describes the genomes of prokaryotes, eukaryotes, vertebrates, and humans; reviews methods for genome sequencing, phenotype data collection, homology searches and analysis, and phylogenetic tree and network building; discusses databases of genome sequences and related information, evolutionary distances, and population genomics; provides supplementary material at an associated website.